Work

Preparing EV-depleted media v1

Year: 2020

Type: preprint

Abstract: This protocol describes a method to prepare bovine EV-depleted 10% FBS cell culture media by ultracentrifugation of 20% FBS media and subsequent dilution.

DOI: 10.17504/protocols.io.biihkcb6

Authors: Aizea Morales Kastresana, Bryce Killingsworth, Joshua A Welsh, Tim Traynor, Jennifer Jones

Institutions: National Institutes of Health, National Cancer Institute, National Cancer Institute, Center for Cancer Research

Language: English

Topic: Extracellular vesicles in disease

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

OpenAlex ID: W3087297372

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