Comparing the Potency, Stability and Cell Stimulating Activity of Protegrin-1 with a Hybrid Antimicrobial Peptide
Year: 2020
Type: dissertation
Abstract: Multi-drug resistant (MDR) bacteria and their biofilms are a concern in veterinary and human medicine. Protegrin-1 (PG-1) is a potent antimicrobial peptide (AMP) with immunomodulatory properties, but its application as a therapeutic is limited by its instability in physiological conditions. To improve stability of PG-1, a hybrid peptide, SynPG-1, was designed. The aim of this study was to compare these two peptides in three categories: antimicrobial and antibiofilm activity against MDR pathogens; stability within physiological fluids; and impact on IPEC-J2 cell line. The results show that SynPG-1 demonstrated a 12-fold increase in serum stability from PG-1 and retained activity after 24hrs of pre-incubation. Both peptides marginally inhibited formation of biofilms and reduction of biomass in pre-formed biofilms, but showed substantial reduction in bacteria within biofilms. Peptides maintained activity after incubation with trypsin and porcine gastric fluid, but not intestinal fluid, and were able to stimulate IPEC-J2 cell migration.
Author: Anna Maystrenko
Language: English
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3087693843