Year: 2020
Type: article
Abstract: A major challenge in three-dimensional (3D) bioprinting is the limited number of bioinks that fulfill the physicochemical requirements of printing while also providing a desirable environment for encapsulated cells. Here, we address this limitation by temporarily stabilizing bioinks with a complementary thermo-reversible gelatin network. This strategy enables the effective printing of biomaterials that would typically not meet printing requirements, with instrument parameters and structural output largely independent of the base biomaterial. This approach is demonstrated across a library of photocrosslinkable bioinks derived from natural and synthetic polymers, including gelatin, hyaluronic acid, chondroitin sulfate, dextran, alginate, chitosan, heparin, and poly(ethylene glycol). A range of complex and heterogeneous structures are printed, including soft hydrogel constructs supporting the 3D culture of astrocytes. This highly generalizable methodology expands the palette of available bioinks, allowing the biofabrication of constructs optimized to meet the biological requirements of cell culture and tissue engineering.
Source: Science Advances
Institutions: Imperial College London, University of Pennsylvania
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $4,500
Awards: G2348666761, Engineering vascularised and aligned tissues using ultrasound cell patterning, G3797080459, G4129834143, G4465225751, G5637376001, Exploring and Engineering the Cell-Material Interface for Regenerative Medicine., G6818991556, Engineering growth factor microenvironments - a new therapeutic paradigm for regenerative medicine, Development of New Biomaterials for Regenerative Medicine, G8651822257
OpenAlex ID: W3087699589