Year: 2019
Type: dissertation
Abstract: Our genome is packaged by nucleosomes consisting of ~147 base pairs of DNA wrapped around a histone octamer. Nucleosomes carry post-translational modifications (PTMs) on their tails which regulates our chromatin biology. During replication, nucleosomal histones, termed ‘parental’ histones, segregate from the parental DNA strand onto the nascent DNA strands. To maintain histone density, parental histones are deposited with new histones lacking epigenetic marks and act as a template to ensure the appropriate PTMs are transmitted on new histones. How parental histones are segregated and re-deposited on nascent DNA is not fully elucidated. I hypothesized that by profiling a known component of the parental histone segregation pathway, the Minichromosome Maintenance 2 (MCM2) protein, with or without its histone binding domain (HBD) I can identify components of this pathway. Here, using a proximity-dependent labelling technique, BioID, I identified 6 candidate proteins in the parental histone segregation pathway.
Source: TSpace (University of Toronto)
Author: Stephanie Tran
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3089436839