Histamine is the Macrophage-derived Factor that Plays a Crucial Role in Promoting Axon Regeneration of Retinal Explants
Year: 2020
Type: dissertation
Abstract: The role of macrophages in promoting axon regeneration in the injured mammalian CNS is still strongly debated. While there have been past studies that reported regenerative effects induced by inflammation following injuries to the lens or the optic nerve, there are still several gaps in our understanding of the underlying mechanisms behind achieving that successful axon regeneration. With the use of three different primary cell lines, we show that histamine is the active macrophage-derived factor that is responsible for neurite growth-promoting effects observed in the retinal explant cultures. We also report that histamine is mediating these effects by binding to the H1 receptors found on the astroglial cells. Furthermore, our findings indicate that histamine is influencing the exosome secretion from astroglial cells in eliciting these effects. Therefore, our results address the role of macrophages and show the mechanism whereby they are promoting the axon regrowth at the CNS injury site.
Source: TSpace (University of Toronto)
Author: Khyati Walia
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3090801994