Reply to van Swinderen and Hines: Drosophila model establishes the lipid membrane as a target of anesthetics
Year: 2020
Type: letter
Abstract: We thank van Swinderen and Hines (1) for their response to our recent article, where we find that anesthetics disrupt lipid rafts and activate phospholipase D (PLD) through a membrane-mediated mechanism (2). Here we take the opportunity to address some minor misunderstandings and clarify our experimental procedures. Our chloroform induction in Drosophila is part of a larger study (3) which we reference in our study (reference 40 in ref. 2), referring the reader to a detailed description of the animal experiments, including the genotype of the wild-type flies (w1118), and full dose–response curves for three anesthetics (chloroform, isoflurane, … [↵][1]1To whom correspondence may be addressed. Email: shansen{at}scripps.edu or rlerner{at}scripps.edu. [1]: #xref-corresp-1-1
Authors: Scott B. Hansen, Richard A. Lerner, Mahmud Arif Pavel, E. Nicholas Petersen
Institution: Scripps Research Institute
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3091201559