Year: 2020
Type: article
Abstract: Non-DNA labels are key components for the construction of functional DNA nanostructures. Here, we present a method to graft covalent labels onto DNA origami nanostructures in an enzymatic one-pot reaction. The DNA methyltransferase M.TaqI labels the DNA nanostructures with azide groups, which serve as universal attachment points via click chemistry. Direct labeling with fluorescent dyes is also demonstrated. The procedure yields structures with high fluorescence intensities and narrow intensity distributions. In combination with UV crosslinking it enables the creation of temperature-stable, intense fluorescent beacons.
Source: Nanoscale
DOI: 10.1039/d0nr03694c
Institutions: Tel Aviv University, RWTH Aachen University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Awards: G1649609151, Exploring the Epigenome by Multiplexed Physical Mapping of Individual Chromosomes, Verbundprojekt: Lab-on-a-Chip Krebsdiagnose durch Quantifizierung von epigenetischen Markern (CancerEpiQuant) - Teilvorhaben: Entwicklung orthogonaler chemo-enzymatischer Markierungsreaktionen für die Fluoreszenzdetektion epigenetischer DNA-Modifikationen
OpenAlex ID: W3091339440