Year: 2020
Type: review
Abstract: The kidneys are essential organs that filter the blood, removing urinary waste while maintaining fluid and electrolyte homeostasis. Current conventional research models such as static cell cultures and animal models are insufficient to grasp the complex human in vivo situation or lack translational value. To accelerate kidney research, novel research tools are required. Recent developments have allowed the directed differentiation of induced pluripotent stem cells to generate kidney organoids. Kidney organoids resemble the human kidney in vitro and can be applied in regenerative medicine and as developmental, toxicity, and disease models. Although current studies have shown great promise, challenges remain including the immaturity, limited reproducibility, and lack of perfusable vascular and collecting duct systems. This review gives an overview of our current understanding of nephrogenesis that enabled the generation of kidney organoids. Next, the potential applications of kidney organoids are discussed followed by future perspectives. This review proposes that advancement in kidney organoid research will be facilitated through our increasing knowledge on nephrogenesis and combining promising techniques such as organ-on-a-chip models.
Source: Journal of Molecular Medicine
Authors: Navin Gupta✉, Emre Dilmen, Ryuji Morizane
Language: English
Topic: Renal and related cancers
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,860
Awards: Kidney Microphysiological Analysis Platforms (MAP) to Optimize Function and Model Disease, Ex Vivo Generation of Functional Kidney Tissues for Transplantation, Biomanufacturing of Vascularized Kidney Tissues: A Foundational Step towards Building a Kidney, Recreating Kidney Organogenesis in vitro with Human Pluripotent Stem Cells, Vascularized kidney organoids on chip for efficacy and toxicity testing of somatic genome editing, G5274089326, Vascularized kidney organoids on chip for efficacy and toxicity testing of somatic genome editing, Kidney Microphysiological Analysis Platforms (MAP) to Optimize Function and Model Disease
OpenAlex ID: W3091779710