Effect of Duration of Cold Pretreatment and Plant Growth Regulators on Callusing and Green Plant Regeneration of Indica (Oryza sativa L.) Rice
Year: 2020
Type: article
Abstract: Improvement of indica rice lines is a challenging task through application of anther culture as they are recalcitrant to culture distinctive to japonica rices. Current investigation aimed to verify the effect of cold pretreatment and phytohormones on the anther culture response of 6 crosses. Cold pretreatment for 7-10 days at 100C was found to have profound effect on callus induction frequency irrespective of the media employed and the prolonged treatment over the optimum showed to be inhibitory. Of the different auxins (2,4-D, NAA) and cytokinins (Kinetin, BAP)and their combinations studied, a ratio of 1:4 for 2,4-D and NAA and 1:3:1 ratio of Kinetin: BAP: NAA ratio proved to be optimal for callus induction and green plant regeneration respectively. This information suggests the opportunity of exploiting hybrid vigor in indica rice cultivars for the development of doubled haploids with more yield and yield attributes from elite hybrid crosses.
Authors: Muntazir Mushtaq, Anil Kumar Singh, Romesh Kumar Salgotra, Manmohan Sharma, R. K. Samnotra, Vikas Sharma, Sunali Mahajan, M. Iqbal Jeelani Bhat, Rizwan Yousuf
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W3092066719