Work

A CRISPR Interference Platform for Selective Downregulation of Gene Expression in Borrelia burgdorferi

Year: 2020

Type: article

Abstract: Gene function studies are facilitated by the availability of rapid and easy-to-use genetic tools. Homologous recombination-based methods traditionally used to genetically investigate gene function remain cumbersome to perform in B. burgdorferi , as they often are relatively inefficient.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.02519-20

Authors: Constantin N. Takacs, Molly Scott, Yunjie Chang, Zachary A. Kloos, Irnov Irnov, Patricia A. Rosa, Jun Liu, Christine Jacobs-Wagner

Institutions: Howard Hughes Medical Institute, Yale University, Stanford University, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: Howard Hughes Medical Institute, American Heart Association, Yale University, National Institutes of Health, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases

Awards: G2825672826, Medical Scientist Training Program, Genome organization and segregation in the spirochete bacterium Borrelia burgdorferi, the agent of Lyme carditis., Delineation of unique flagellar proteins in spirochetes, Structure-Function Relationships in the Spirochetal Flagellar Motor

OpenAlex ID: W3108620378

FWCI: 1.07

Cites: 92

Cited by: 27

Related to: 10