Clustered rapid induction of apoptosis limits ZIKV and DENV-2 proliferation in the midguts of Aedes aegypti
Year: 2021
Type: article
Abstract: ABSTRACT Inter-host transmission of pathogenic arboviruses such as dengue virus (DENV) and Zika virus (ZIKV) requires systemic infection of the mosquito vector. Successful systemic infection requires initial viral entry and proliferation in the midgut cells of the mosquito followed by dissemination to secondary tissues and eventual entry into salivary glands 1 . Lack of arbovirus proliferation in midgut cells has been observed in several Aedes aegypti strains 2 , but the midgut antiviral responses underlying this phenomenon are not yet fully understood. We report here that there is a rapid induction of apoptosis (RIA) in the Aedes aegypti midgut epithelium within 2 hours of infection with DENV-2 or ZIKV in both in vivo blood-feeding and ex vivo midgut infection models. Inhibition of RIA led to increased virus proliferation in the midgut, implicating RIA as an innate immune mechanism mediating midgut infection in this mosquito vector.
Source: Communications Biology
Institution: University of Florida
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,290
Funders: U.S. Department of Health and Human Services, U.S. Department of Agriculture, University of Florida, National Institutes of Health, Centers for Disease Control and Prevention, Emerging Pathogens Institute, University of Florida, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Center for Emerging and Zoonotic Infectious Diseases
OpenAlex ID: W3122060777