Occludin, caveolin‐1, and Alix form a multi‐protein complex and regulate HIV‐1 infection of brain pericytes
Year: 2020
Type: article
Abstract: HIV-1 enters the brain by altering properties of the blood-brain barrier (BBB). Recent evidence indicates that among cells of the BBB, pericytes are prone to HIV-1 infection. Occludin (ocln) and caveolin-1 (cav-1) are critical determinants of BBB integrity that can regulate barrier properties of the BBB in response to HIV-1 infection. Additionally, Alix is an early acting endosomal factor involved in HIV-1 budding from the cells. The aim of the present study was to evaluate the role of cav-1, ocln, and Alix in HIV-1 infection of brain pericytes. Our results indicated that cav-1, ocln, and Alix form a multi-protein complex in which they cross-regulate each other's expression. Importantly, the stability of this complex was affected by HIV-1 infection. Modifications of the complex resulted in diminished HIV-1 infection and alterations of the cytokine profile produced by brain pericytes. These results identify a novel mechanism involved in HIV-1 infection contributing to a better understanding of the HIV-1 pathology and the associated neuroinflammatory responses.
Source: The FASEB Journal
Institution: University of Miami
Language: English
Subfield: Neurology
Field: Neuroscience
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $2,500
Awards: G1015249216, G1056143923, G1309373569, G140693692, G1638005703, G2271813062, G3117022776, G3616991090, G4395712398, G4669688211, G5164113070, G5775888695, G5943719250, A new pathway of spinal neurons in neuropathic pain induced by HIV with opioid, G628684116, G7005395014, G7068466250, G7168689909, G7224178199, Targeting Inflammasomes in Substance Abuse and HIV, G7822443850, Novel mechanisms of enhanced stroke susceptibility in HIV-infected brain, Nano-delivery of methanandamide across BBB to block cannabinoid induced effects in HIV-1 infection, Role of the BBB in HIV brain infection during methamphetamine abuse, G8556416181, Interplay between cArt, HIV, and amyloid at the blood-brain barrier., G8915390089, HIV-1 and amyloid beta interactions at the blood-brain barrier, Defining brain pericytes as a novel and myeloid-derived HIV reservoir
OpenAlex ID: W3092855613