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Protein Thermodynamic Stability: On‐Chip Acousto Thermal Shift Assay for Rapid and Sensitive Assessment of Protein Thermodynamic Stability (Small 41/2020)

Year: 2020

Type: article

Abstract: In article number 2003506, Xiaoyun Ding and co-workers present an acousto thermal shift assay (ATSA) utilizing acoustic mechanisms to denature proteins and concentrate protein precipitates for rapid and label-free assessment of protein thermodynamic changes in real time. The whole process, including sample handling, protein unfolding, and measurement, takes place within a microfluidic chip in less than two minutes, and is faster than other current thermal shift assays.

Source: Small

DOI: 10.1002/smll.202070224

Authors: Yonghui Ding, Kerri A. Ball, Kristofor J. Webb, Yu Gao, Angelo D'Alessandro, William M. Old, Michael H. B. Stowell, Xiaoyun Ding

Institutions: University of Colorado Boulder, University of Colorado System, University of Colorado Anschutz

Language: English

Topic: thermodynamics and calorimetric analyses

Subfield: Physical and Theoretical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: bronze

OpenAlex ID: W3093444597

FWCI:

Cites: 1

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Related to: 10