Matrix vesicle biomimetics carrying Annexin A5 and Alkaline Phosphatase bind to native collagen produced by human smooth muscle cell transdifferentiated in osteo/chondrocyte cells
Year: 2020
Type: article
Abstract: Background: Osteocytes differentiate from cuboidal-like osteoblasts and are embedded into soft secreted osteoid in mature bone.The regulation of osteocyte differentiation remains poorly understood, however, collagen as a scaffold will have an influence on it.At the ECTS2019 Congress, we showed that low adhesive scaffold type I collagen (LASCol) has marvelous ability to induce from mesenchymal stromal cells (MSCs) to osteoblasts differentiation in short term.In this study, we report the effects of LASCol to promote differentiation from MSCs into osteocyte-like cells followed after osteoblasts.Methods: Rat MSCs were cultured on LASCol coated-dish or LASCol gel with osteogenic basal medium.The alkaline phosphatase (ALP) and Alizarin red staining of rMMCs cultured on the LASCol coated dish was observed.The expression of mRNAs related to osteocytes differentiation was analysed with qRT-PCR.In addition, Transmission Electron Microscope (TEM) was used to observe the features of cells in LASCol gel.To investigate local properties of the LASCol gel surface, we measured stiffness of LASCol gel by Scanning Probe Microscope (SPM) and surface images were also acquired.Results: On both the LASCol coated-dish and the LASCol gel, rat MSCs formed spheroid bodies and highly mineralized extracellular matrix was indicated.After 3 and 5 days culture, the osteocyte-related genes such as Bglap, Dmp1, Spp1, and Phex significantly increased (2.4b ΔΔCtb 231).Interestingly, we confirmed osteocyte-like cells in LASCol gel after 40 days culture.After incubation for 1h at 37°C for gel formation, the stiffness of LASCol gel showed an increase depending on concentration (6.8 nN at 10.5 mg/mL).Probably, LASCol would have the strong ability to induce the differentiation of from rMSCs to osteocyte.
Source: Bone Reports
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,450
OpenAlex ID: W3093530380