Work

Biodynamic digital holographic speckle microscopy for oocyte and embryo metabolic evaluation

Year: 2020

Type: article

Abstract: Assisted reproductive technologies seek to improve the success rate of pregnancies. Morphology scoring is a common approach to evaluate oocyte and embryo viability prior to embryo transfer in utero, but the efficacy of the method is low. We apply biodynamic imaging, based on dynamic light scattering and low-coherence digital holography, to assess the metabolic activity of oocytes and embryos. A biodynamic microscope, developed to image small and translucent biological specimens, is inserted into the bay of a commercial inverted microscope that can switch between conventional microscopy channels and biodynamic microscopy. We find intracellular Doppler spectral features that act as noninvasive proxies for embryo metabolic activity that may relate to embryo viability.

Source: Applied Optics

DOI: 10.1364/ao.404298

Authors: Zhe Li, Ilka M. Lorenzo-Lorenzo, Ran An, John Turek, David D. Nolte, Zoltan Machaty

Institution: Purdue University West Lafayette

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: green

Funders: Directorate for Engineering, Eunice Kennedy Shriver National Institute of Child Health and Human Development

Awards: Assessment of the developmental potential of oocytes and embryos using biodynamic imaging, G8637749427