Year: 2020
Type: article
Abstract: Metabolomics plays a pivotal role in systems biology, and NMR is a central tool with high precision and exceptional resolution of chemical information. Most NMR metabolomic studies are based on 1H 1D spectroscopy, severely limited by peak overlap. 13C NMR benefits from a larger signal dispersion but is barely used in metabolomics due to ca. 6000-fold lower sensitivity. We introduce a new approach, based on hyperpolarized 13C NMR at natural abundance, that circumvents this limitation. A new untargeted NMR-based metabolomic workflow based on dissolution dynamic nuclear polarization (d-DNP) for the first time enabled hyperpolarized natural abundance 13C metabolomics. Statistical analysis of resulting hyperpolarized 13C data distinguishes two groups of plant (tomato) extracts and highlights biomarkers, in full agreement with previous results on the same biological model. We also optimize parameters of the semiautomated d-DNP system suitable for high-throughput studies.
Source: Analytical Chemistry
Authors: Arnab Dey, Benoît Charrier, Estelle Martineau, Catherine Deborde, Elodie Gandriau, Annick Moing, Daniel Jacob, Dmitry Eshchenko, Marc Schnell, Roberto Melzi, Dennis Kurzbach, Morgan Ceillier, Quentin Chappuis, Samuel F. Cousin, James G. Kempf, Sami Jannin, Jean-Nicolas Dumez, Patrick Giraudeau
Institutions: Centre National de la Recherche Scientifique, Chimie et Interdisciplinarité, Synthèse, Analyse, Modélisation, Université de Nantes, Nantes Université, Université de Bordeaux, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Biologie du Fruit et Pathologie, MetaboHUB, Bruker (Switzerland), University of Vienna, Lyon 1 Université, École Normale Supérieure de Lyon, Université de Lyon, Centre de RMN à Très Hauts Champs de Lyon, Bruker (United States)
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $5,000
Awards: NExT (I-SITE), Site-specific Ultrasensitive Magnetic resonance of Mixtures for Isotopic Tracking, Hyperpolarized Nuclear Magnetic Resonance Spectroscopy for Time-Resolved Monitoring of Interactions of Intrinsically Disordered Breast-Cancer Proteins, Persistent and Transportable Hyperpolarization for Magnetic Resonance, Real-time diffusion NMR analysis of mixtures, Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation
OpenAlex ID: W3097039690