Work

Establishment of screening method for salt tolerant soybean at emergence stage and screening of tolerant germplasm

Year: 2019

Type: article

Abstract: : Salinity is an important factor affecting crop production. Screening salt tolerant soybean germplasm is of great significance for the utilization of salinized soil in major soybean production regions. In order to select salt tolerant soybean, a screening method was developed by using six soybean accessions, including Zhonghuang 35, Zhonghuang 39, Williams 82, Tiefeng 8, Peking, and NY27-38. Seeds were grown in vermiculite and treated with 0, 100, and 150 mmol L-1 NaCl solution. Seedling rate (SR), plant height (H), fresh weight of shoot and root (FWS and FWR), dry weight of shoot and root (DWS and DWR) were decreased significantly under 150 mmol L-1 NaCl treatment, with significant difference among varieties. Therefore, 150 mmol L-1 NaCl was suitable to identify salt tolerant soybean at emergence stage. The salt tolerance index (SI) based on the growth and development of seedlings and the salt tolerance coefficient (ST) were significantly correlated with the salt tolerance. The method using salt tolerance index is non-destructive and does not require planting control, which could save time and labor in salt tolerant germplasm identification. Twenty-seven soybean resources were screened, in which three were highly tolerant (grade 1) and seven tolerant (grade 2) at emergence stage. Among them, Yundou 101, Zheng 1311, Wansu 1015, and Tiefeng 8 also showed salt tole at emergence stage. Among them, Yundou 101, Zheng 1311, Wansu 1015, and Tiefeng 8 also showed salt tolerance (grade 1) at seedling stage. In summary, an effective method for screening salt tolerant soybean at emergence stage was developed, with vermiculite as the substrate, 150 mmol L-1 NaCl as suitable treatment solution, and salt tolerance index as the indicator. Four soybean accessions were found to be salt tolerant at both emergence and seedling stages. This screening method will be useful for identification of salt tolerant soybean germplasm.

Source: ACTA AGRONOMICA SINICA

DOI: 10.3724/sp.j.1006.2020.94062

Authors: Xie-Xiang LIU, Ru-Zhen CHANG, Rong-Xia GUAN, Li-Juan QIU

Institutions: Chinese Academy of Agricultural Sciences, Institute of Crop Sciences

Language: English

Topic: Plant Stress Responses and Tolerance

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

Funder: National Natural Science Foundation of China