Year: 2020
Type: article
Abstract: DNA origami, in which a long scaffold strand is assembled with a many short staple strands into parallel arrays of double helices, has proven a powerful method for custom nanofabrication. However, currently the design and optimization of custom 3D DNA-origami shapes is a barrier to rapid application to new areas. Here we introduce a modular barrel architecture, and demonstrate hierarchical assembly of a 100 megadalton DNA-origami barrel of ~90 nm diameter and ~250 nm height, that provides a rhombic-lattice canvas of a thousand pixels each, with pitch of ~8 nm, on its inner and outer surfaces. Complex patterns rendered on these surfaces were resolved using up to twelve rounds of Exchange-PAINT super-resolution microscopy. We envision these structures as versatile nanoscale pegboards for applications requiring complex 3D arrangements of matter, which will serve to promote rapid uptake of this technology in diverse fields beyond specialist groups working in DNA nanotechnology.
Source: Nature Communications
Authors: Shelley F. J. Wickham, Alexander Auer, Jianghong Min, Nandhini Ponnuswamy, Johannes B. Woehrstein, Florian Schueder, Maximilian T. Strauss, Jörg Schnitzbauer, Bhavik Nathwani, Zhao Zhao, Steven D. Perrault, Jaeseung Hahn, Seungwoo Lee, Maartje M. Bastings, Sarah W. Helmig, Anne Louise Kodal, Peng Yin, Ralf Jungmann, William M. Shih
Institutions: The University of Sydney, Harvard University, Dana-Farber Cancer Institute, Wyss Institute for Biologically Inspired Engineering, Center for NanoScience, Max Planck Institute of Biochemistry, Ludwig-Maximilians-Universität München, Aarhus University, Danish National Research Foundation
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,380
Funders: National Science Foundation, U.S. Department of Defense, International Max Planck Research School for Environmental, Cellular and Molecular Microbiology, National Research Foundation, Deutsche Forschungsgemeinschaft, Danmarks Grundforskningsfond, Max-Planck-Gesellschaft, International Max Planck Research School for Advanced Methods in Process and Systems Engineering, Hansjörg Wyss Institute for Biologically Inspired Engineering, Harvard University, Australian Research Council, Army Research Office
Awards: G1242026511, G1566101025, G1586008684, DNA origami functionalised nanoscale surfaces, G295756641, G4278370412, Collaborative Research: Molecular Programming Architectures, Abstractions, Algorithms, and Applications, TRANSLATING BIOCHEMICAL PATHWAYS TO NON-CELLULAR ENVIRONMENT, G6413502617, Center for Cellular Signal Patterns (CellPAT), G7452299184, G8421151815
OpenAlex ID: W3100906359