Year: 2017
Type: article
Abstract: High-resolution Bragg diffraction from aerosolized single granulovirus nano-crystals using an X-ray free-electron laser is demonstrated. The outerdimensions of the in-vacuum aerosol injector components are identical toconventional liquid-microjet nozzles used in serial diffraction experiments,which allows the injector to be utilized with standard mountings. As comparedwith liquid-jet injection, the X-ray scattering background is reduced by severalorders of magnitude by the use of helium carrier gas rather than liquid. Suchreduction is required for diffraction measurements of small macromolecularnanocrystals and single particles. High particle speeds are achieved, making theapproach suitable for use at upcoming high-repetition-rate facilities.
Authors: Awel, Salah, Kirian, Richard A., Adriano, Luigi, Tolstikova, Alexandra, Paulraj, Lourdu Xavier, Yefanov, Oleksandr, Aquila, Andrew, Barty, Anton, Roy-Chowdhury, Shatabdi, Hunter, Mark S., James, Daniel, Robinson, Joseph S., Wiedorn, Max O., Weierstall, Uwe, Rode, Andrei, Bajt, Saša, Küpper, Jochen, Chapman, Henry N., Beyerlein, Kenneth, Roth, Nils, Horke, Daniel, Oberthür, Dominik, Knoska, Juraj, Mariani, Valerio, Morgan, Andrew
Institutions: Universität Hamburg, Deutsches Elektronen-Synchrotron DESY, Center for Free-Electron Laser Science, Hamburg Centre for Ultrafast Imaging, Arizona State University, SLAC National Accelerator Laboratory, Linac Coherent Light Source, Max Planck Institute for the Structure and Dynamics of Matter, Australian National University
Language: English
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: green
Estimated APC paid: $1,141
Funders: National Science Foundation, U.S. Department of Energy, European Commission, Helmholtz-Gemeinschaft, Deutsche Forschungsgemeinschaft, National Institutes of Health, Office of Science, Australian Research Council, Deutsches Elektronen-Synchrotron, National Institute of General Medical Sciences, National Center for Research Resources, Basic Energy Sciences, SLAC National Accelerator Laboratory
Awards: G1229526393, G1286251239, G1307938796, G1926397799, G2371548206, G2407759970, G262532246, G303061748, G4235786860, A Synchroton Radiation Structural Biology Resource, G4908923434, G495301720, G498139845, Nanometrology of laser-trapped airborne particles, G5570207115, G628751139, G6550344761, Non-equilibrium material phases, G7083997402, G7283024673, G7313278570, G8382000481, A Synchrotron Radiation Structural Biology Resource, G911061179, Biology with X-ray Lasers, G969889393
OpenAlex ID: W3101278905