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The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation

Year: 2023
Type: article
Abstract: Sleep is a nearly universal feature of animal behaviour, yet many of the molecular, genetic, and neuronal substrates that orchestrate sleep/wake transitions lie undiscovered. Employing a viral insertion sleep screen in larval zebrafish, we identified a novel gene, dreammist ( dmist ), whose loss results in behavioural hyperactivity and reduced sleep at night. The neuronally expressed dmist gene is conserved across vertebrates and encodes a small single-pass transmembrane protein that is structurally similar to the Na + ,K + -ATPase regulator, FXYD1/Phospholemman. Disruption of either fxyd1 or atp1a3a , a Na + ,K + -ATPase alpha-3 subunit associated with several heritable movement disorders in humans, led to decreased night-time sleep. Since atpa1a3a and dmist mutants have elevated intracellular Na + levels and non-additive effects on sleep amount at night, we propose that Dmist-dependent enhancement of Na + pump function modulates neuronal excitability to maintain normal sleep behaviour.
Source: eLife
DOI: 10.7554/elife.87521.3
Authors: Ida L. Barlow, Eirinn Mackay, Emily Wheater, Aimee Goel, Sumi Lim, Steve Zimmerman, Ian Woods, David A. Prober, Jason Rihel
Institutions: MRC London Institute of Medical Sciences, University College London, Imperial College London, Sainsbury Wellcome Centre, MRC Centre for Reproductive Health, University of Edinburgh, Harvard University, Ithaca College, California Institute of Technology
Language: English
Open Access status: gold
Estimated APC paid: $2,500
Funders: Harvard University, Wellcome Trust, National Institutes of Health, European Research Council, National Institute of General Medical Sciences, National Institute of Neurological Disorders and Stroke, FP7 Ideas: European Research Council
OpenAlex ID: W4375949141
FWCI: 1.282
Cites: 101
Cited by: 13
Related to: 9