Year: 2020
Type: article
Abstract: Introduction: The wide-ranging positive effects of phytoecdisteroids suggest use them as drugs for the treatment of diabetes and related diseases.Among phytoecdysteroids, a well-expressed hypoglycemic effect is known for turke sterone.Objective: We aimed to determine the effect of turke sterone on the endocrine and exocrine parameters of the pancreas in alloxan-induced diabetic rats.Material and Methods: White outbred rats were divided into 4 groups to receive saline or a single dose of alloxan (170 mg/kg, intraperitoneal) and/or turkesterone (10 mg/kg/24 h) and glibenclamide (5 mg/kg/24 h) for 10 days.For histological analysis the tail portion of the pancreas was kept in formalin for 3 days, dried in ethanol, parafinized and then stained with hematoxylin-eosin.The glucose, insulin, C-peptide and total protein level in serum, as well as α-amylase activity in serum and pancreas homogenate, were identified by using special kits (Human.Germany).Results: Diabetes caused complete destruction of the islet apparatus and partial destruction of the pancreatic acini tissue.Treatment with turkesterone of alloxan-induced diabetic rats resulted in restoration of the histostructure of endo-and exocrine pancreas, increase of serum glucose as well as decrease insulin, C-peptide, and total protein level.In addition, turkesterone treatment of alloxan-induced diabetic rats led to an approximation of α-amylase activity of pancreas and serum to control value. Conclusion:This study has shown that turkesterone led to improving the exocrine and endocrine function in alloxan-indused diabetic rats.Hence, it can be recommended for further testing as an anti-pancreatic and anti-diabetic drug in humans.
Authors: Kuchkarova LS, Rokhimova SO, Syrov VN
Language: English
Subfield: Endocrinology, Diabetes and Metabolism
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W3105003510