Work

Generation of a Single-Cell RNAseq Atlas of Murine Salivary Gland Development

Year: 2020

Type: article

Abstract: Understanding the dynamic transcriptional landscape throughout organ development will provide a template for regenerative therapies. Here, we generated a single-cell RNA sequencing atlas of murine submandibular glands identifying transcriptional profiles that revealed cellular heterogeneity during landmark developmental events: end bud formation, branching morphogenesis, cytodifferentiation, maturation, and homeostasis. Trajectory inference analysis suggests plasticity among acinar and duct populations. We identify transcription factors correlated with acinar differentiation including Spdef, Etv1 , and Xbp1 , and loss of Ybx1 , Eno1, Sox11, and Atf4 . Furthermore, we characterize two intercalated duct populations defined by either Gfra3 and Kit , or Gstt1 . This atlas can be used to investigate specific cell functions and comparative studies predicting common mechanisms involved in development of branching organs.

Source: iScience

DOI: 10.1016/j.isci.2020.101838

Authors: Belinda R. Hauser, Marit H. Aure, Michael C. Kelly, Matthew P. Hoffman, Alejandro M. Chibly

Institutions: National Institutes of Health, National Institute of Dental and Craniofacial Research

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,000

Funders: National Institutes of Health, National Institute on Deafness and Other Communication Disorders, National Institute of Dental and Craniofacial Research