Work

Analysis of Primary Cilia in Rodent Brain By Immunofluorescence Microscopy v1

Year: 2020

Type: preprint

Abstract: We describe here our method for immunostaining of primary cilia in brain sections from wild type and LRRK2 or other mutant mice. Included are procedures for perfusion, tissue harvesting, sectioning and staining, image acquisition and analysis.

DOI: 10.17504/protocols.io.bnwimfce

Authors: Shahzad S. Khan, Herschel S. Dhekne, Francesca Tonelli, Suzanne R. Pfeffer

Institutions: MRC Protein Phosphorylation and Ubiquitylation Unit, Stanford Medicine, Stanford University

Language: English

Topic: Genetic and Kidney Cyst Diseases

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W3110203380

Cites:

Cited by: 2

Related to: 10