Hormonal Regulation of Cell Proliferation and Differentiation of Vigna sinensis Callus in Vitro (A. NATURAL SCIENCE)
Year: 1983
Type: article
Abstract: The growth of Vigna sinensis callus was promoted synergistically by auxin, cytokinin and nicotinic acid. Strong auxins such as 2,4,5-T (2,4,5-trichlorophenoxyacetic acid), 2,4-D (2,4-dichlorophenoxyacetic acid) and NAA (α-naphthaleneacetic acid) at low concentration (1 or 0.1μM) stimulated cell proliferation, but inhibited root formation. High concentration (22.1μM) of 2,4-D caused watery friable callus composed of free round vacuolated and undifferentiated cells. On the other hand, an addition of kinetin decreased free cell formation and gave compact tissue. High level (10 and 40μM) of kinetin induced cell differentiation to tracheal element, but 40μM kinetin rather inhibited formation of tracheal element in the case of low level (0 or 0.0221μM) of 2,4-D. Thus, relative amounts of auxins and cytokinins determined cell characteristics and differentiation. Nicotinic acid had no effect on tissue texture, cell characteristics and differentiation, although stimulated strongly cell proliferation.
Author: Satoshi Matsubara
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W3110512374