Iron uptake mediated by the plant-derived chelator nicotianamine in the small intestine
Year: 2021
Type: article
Abstract: Iron is an essential metal for all living organisms that is absorbed in the intestinal cells as a heme-chelated or free form. It is unclear how important plant-derived chelators, such as nicotianamine (NA), an organic small molecule that is ubiquitous in crops, vegetables, and various other foods, contribute to iron bioavailability in mammals. We performed electrophysiological assays with Xenopus laevis oocytes and radioactive tracer experiments with Caco-2 cells. The findings revealed that the proton-coupled amino acid transporter SLC36A1 (PAT1) transports iron in the form of NA-Fe (II) complex in vitro . Decreased expression of hPAT1 by RNA interference in Caco-2 cells reduced the uptake of NA- 59 Fe (II) complex. The uptake of inorganic 59 Fe (II) was relatively unaffected. These results imply that PAT1 transports iron as a NA-Fe (II) complex. The rate of 59 Fe absorption in the spleen, liver, and kidney was higher when mice were orally administered NA- 59 Fe (II) compared with free 59 Fe (II). The profile of site-specific PAT1 expression in the mouse intestine coincided with those of NA and iron contents, which were the highest in the proximal jejunum. Orally administered NA- 59 Fe (II) complex in mice was detected in the proximal jejunum by thin layer chromatography. In contrast, much less 59 Fe (or NA) was detected in the duodenum, where the divalent metal transporter SLC11A2 (DMT1) absorbs free Fe (II). The collective results revealed the role of PAT1 in NA-Fe (II) absorption in the intestine and potential implication of NA in iron uptake in mammals.
Source: Journal of Biological Chemistry
Institutions: Suntory Foundation for Life Sciences, Tokushima University
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $2,500
Awards: Effects of iron chelator nicotianamine from plant food on iron absorption in intestine, Elucidation of molecular mechanism of intestinal iron absorption in mammals by mugineic acid precursor, Elucidation of iron absorption mechanism in mammals by plant-derived iron chelator, the precursor of mugineic acids, Elucidation of the molecular mechanism of the innovative fertilizer that greens the desert: unknown function search of phytosiderophore
OpenAlex ID: W3112798685