Work

Obtaining Human Breast Adipose Cells for Breast Cancer Cell Co-culture Studies

Year: 2020

Type: article

Abstract: Primary human breast cancers invade surrounding fat and contact adipocytes, inflammatory infiltrates, and fibrous stroma. This tissue niche influences breast tumor progression. Here, we present a protocol to enable the in vitro study of the complex interactions that occur between breast cancer cells and adipose cells. We describe how to obtain different adipose cell populations, including adipose-derived stem cells, immature adipocytes, and mature adipocytes, from human breast fat tissue and detail the application for co-culture assays with breast cancer cells. For complete details on the use and execution of this protocol, please refer to Picon-Ruiz et al. (2016) and Qureshi et al. (2020).

Source: STAR Protocols

DOI: 10.1016/j.xpro.2020.100197

Authors: Manuel Picon-Ruiz, Juan A. Marchal, Joyce M. Slingerland

Institutions: Universidad de Granada, Instituto de Investigación Biosanitaria de Granada, Georgetown University, Georgetown Lombardi Comprehensive Cancer Center

Language: English

Topic: Adipose Tissue and Metabolism

Subfield: Physiology (medical)

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550