Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics
Year: 2020
Type: article
Abstract: Clonorchiasis caused by Clonorchis sinensis is endemic in East Asia; approximately 15 million people have been infected thus far. To diagnose the infection, serodiagnostic tests with excellent functionality should be performed. First, 607 expressed sequence tags encoding polypeptides with a secretory signal were expressed into recombinant proteins using an in vitro translation system. By protein array-based screening using C. sinensis-infected sera, 18 antigen candidate proteins were selected and assayed for cross-reactivity against Opisthorchis viverrini-infected sera. Of the six antigenic proteins selected, four were synthesized on large scale in vitro and evaluated for antigenicity against the flukes-infected human sera using ELISA. CsAg17 antigen showed the highest sensitivity (77.1%) and specificity (71.2%). The sensitivity and specificity of the bacterially produced CsAg17-28GST fusion antigen was similar to those of CsAg17 antigen. CsAg17 antigen can be used to develop point-of-care serodiagnostic tests for clonorchiasis.
Source: PLoS neglected tropical diseases
Institutions: Nakdonggang National Institute of Biological Resources, Chung-Ang University, Inha University, Seoul National University, Ehime University, Proteo-Science Center, Ehime University, Kangwon National University, Kyorin University, Khon Kaen University, Korea Association of Health Promotion, Catholic University of Korea, Asan Medical Center, University of Ulsan
Language: English
Subfield: Parasitology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,350
OpenAlex ID: W3114633850