Year: 2020
Type: article
Abstract: We present here the second complete genome of anaerobic ammonium oxidation (anammox) bacterium, Candidatus (Ca.) Brocadia pituitae, along with those of a nitrite oxidizer and two incomplete denitrifiers from the anammox bacterial community (ABC) metagenome. Although NO2- reduction to NO is considered to be the first step in anammox, Ca. B. pituitae lacks nitrite reductase genes (nirK and nirS) responsible for this reaction. Comparative genomics of Ca. B. pituitae with Ca. Kuenenia stuttgartiensis and six other anammox bacteria with nearly complete genomes revealed that their core genome structure contains 1,152 syntenic orthologues. But nitrite reductase genes were absent from the core, whereas two other Brocadia species possess nirK and these genes were horizontally acquired from multiple lineages. In contrast, at least five paralogous hydroxylamine oxidoreductase genes containing candidate ones (hao2 and hao3) encoding another nitrite reductase were observed in the core. Indeed, these two genes were also significantly expressed in Ca. B. pituitae as in other anammox bacteria. Because many nirS and nirK genes have been detected in the ABC metagenome, Ca. B. pituitae presumably utilises not only NO supplied by the ABC members but also NO and/or NH2OH by self-production for anammox metabolism.
Source: DNA Research
Authors: Takashi Okubo, Atsushi Toyoda, Kohei Fukuhara, Ikuo Uchiyama, Yuhki Harigaya, Megumi Kuroiwa, Takuma Suzuki, Yuka Murakami, Yuichi Suwa, Hideto Takami
Language: English
Subfield: Pollution
Field: Environmental Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,400
Awards: Exploring functional differentiation of anammox bacteria: actually diverse anammox, Platform for Advanced Genome Science, Omics reveals nitrogen cycling system with anammox, Comprehending the range of the mangrove rhizosphere - An investigation of plants effects on soil nitrogen dynamics at high spatial resolution, Visualization of the metabolic and physiological potential of microbial community in farmland, Does oxygen supplied to anaerobic soil by mangrove aerial roots facilitate soil nitrate production and plant nitrate use?
OpenAlex ID: W3114900728