Year: 2004
Type: conference-paper
Abstract: We have designed, fabricated, integrated, and tested microfabricated planar patch-clamp substrates and poly(dimethylsiloxane) (PDMS) microfluidic components suitable for use in an automated planar patch-clamp dose-response measurement system.Substrates with cell-patch-site aperture diameters ranging from 300 nm to 12 µm were produced using standard MEMS fabrication techniques.The resistance of the cellpatch sites and substrate capacitance were measured using impedance spectroscopy.The resistance of the cell-patch sites ranged from 200 k to 47 M for apertures ranging from 12 µm to 750 nm respectively.The substrate capacitance was 17.2 pF per mm 2 of fluid contact area for substrates with a 2-µm-thick layer of silicon dioxide.In addition, testing shows that the fluidic components are appropriate for driving human embryonic kidney cells (HEK 293) to patch apertures, for trapping cells on patch apertures, and for rapidly exchanging the extracellular fluid environment.
Authors: B. Matthews, J.W. Judy
Institution: University of California, Los Angeles
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W3118960607