Work

Quantitative Mass Spectrometry Imaging of Biological Systems

Year: 2021

Type: review

Abstract: Mass spectrometry imaging (MSI) is a powerful, label-free technique that provides detailed maps of hundreds of molecules in complex samples with high sensitivity and subcellular spatial resolution. Accurate quantification in MSI relies on a detailed understanding of matrix effects associated with the ionization process along with evaluation of the extraction efficiency and mass-dependent ion losses occurring in the analysis step. We present a critical summary of approaches developed for quantitative MSI of metabolites, lipids, and proteins in biological tissues and discuss their current and future applications.

Source: Annual Review of Physical Chemistry

DOI: 10.1146/annurev-physchem-061020-053416

Authors: Daisy Unsihuay, Daniela Mesa Sanchez, Julia Laskin

Institution: Purdue University West Lafayette

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Heart, Lung, and Blood Institute, National Cancer Institute

Awards: Novel Platform for Quantitative Subcellular Resolution Imaging of Human Tissues Using Mass Spectrometry, Novel Platform for Quantitative Subcellular Resolution Imaging of Human Tissues Using Mass Spectrometry