A genome-wide screen in Saccharomyces cerevisiae identifies Tannic Acid-sensitive mutants
Year: 2021
Type: article
Abstract: We conducted a genome-wide chemical genetics screen of the yeast MATa knockout collection to identify genes that are required for survival on sub-lethal levels of the polyphenol Tannic Acid (TA) (see Fig 1A and reagents). TA, a plant-derived polymeric form of gallic acid connected to a central glucose core, is known to bind and precipitate proteins (Hagerman and Klucher 1986), chelate iron (ferric ions, Fe3), and form TA-Fe3 films on cells, including yeast (Lee et al. 2020). Addition of TA to cultured prostate cancer cells is reported to induce ER stress, increase markers of the unfolded protein response (UPR), and inhibit lipid metabolism (Nagesh et al. 2020). TA has also been reported to act as a potent but non-toxic SUMO E1 inhibitor in several cell lines (Suzawa et al. 2015). How TA induces these effects remains unknown and, therefore, the genome-wide TA-sensitivity screen may provide insights into underlying genetic pathways involved.
Source: PubMed Central
Authors: Emily M Pilc, Shriie Ganesh, Oliver Kerscher
Institution: William & Mary
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3119957852