Year: 2021
Type: letter
Abstract: Glycolysis and gluconeogenesis: A teaching viewJournal of Biological ChemistryVol. 296PreviewI read with interest the recent review “Tracking the carbons supplying gluconeogenesis” by Ankit M. Shah and Fredric E. Wondisford (1). The figures are clear, and they are a good teaching source. Nevertheless, I note some potential teaching issues as well as offer additional suggestions. It is well known and it is explained to our students that there are three reactions of glycolysis that are essentially irreversible: hexokinase, phosphofrutokinase-1, and pyruvate kinase. Thus, I would like to observe in relation to Figure 1 that Full-Text PDF Open Access We thank Félix Hernández for his insightful and thoughtful comments as well as interest in our manuscript (1Shah A.M. Wondisford F.E. Tracking the carbons supplying gluconeogenesis.J. Biol. Chem. 2020; 295: 14419-14429Abstract Full Text Full Text PDF PubMed Scopus (11) Google Scholar). Regarding suggestion 1, we omitted several glycolytic and gluconeogenic enzymes in the figure for simplicity. We specifically mentioned G6Pase and PEPCK as many investigators have studied these enzymes in particular when studying gluconeogenesis. Articles specifically referenced in our review article studied these two enzymes, and we wished to pictorially represent these enzymes. We further described each of these two enzymes’ specific functions in the text. Our manuscript does not go into the regulation of glycolysis or gluconeogenesis as our main intention was to show carbon flux. So we do not feel compelled to label the enzymes as suggested as that is not the intent of the picture. Regarding suggestions 2 and 3, we agree that there should be a single arrow between phosphoenolpyruvate to pyruvate and there should be a single arrow between pyruvate to oxaloacetate to best show carbon flux (Fig. 1). The authors declare that they have no conflicts of interests with the contents of this article.
Source: Journal of Biological Chemistry
Authors: Ankit M. Shah, Fredric E. Wondisford
Institution: Rutgers, The State University of New Jersey
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,500
OpenAlex ID: W3120190520