Work

Shikonin promotes osteogenesis and suppresses osteoclastogenesis in vitro.

Year: 2020

Type: article

Abstract: Shikonin, as a traditional Chinese herbal medicine with a role of anti-cancer, anti-inflammatory, anti-bacterial and other effects. However, there are few studies on the effect of shikonin on osteoporosis. Therefore, the purpose of this study aims to investigate the role and mechanism of shikonin on differentiation of BMSCs and BMMs into osteoblasts and osteoclasts formation. In our study, we treated the cells with different concentrations of shikonin, and then illuminated its effect on oteogenesis and osteoclast differentiation by ALP/alizarin red staining, ALP activity, qRT-PCR, immunofluorescence, Western blot, and TRAP staining. The result showed that shikonin may promote BMSCs differentiate into osteoblasts through the Wnt/β-catenin signaling pathway. At the same time, it may also inhibit the formation of osteoclasts mediated by RANK/RANKL/OPG pathway in vitro. Our research explains excellently the mechanism of shikonin alleviating osteoporosis in vitro, which maybe contributing to the exploration of a new way to prevent osteoporosis.

Source: Europe PMC (PubMed Central)

Authors: Liang Zhou, Jiaqian Wang, Jiali Zhao, Feng Kuai, Huilin Yang

Institutions: First Affiliated Hospital of Soochow University, Second People’s Hospital of Huai’an, Affiliated Hospital of Nantong University, Yancheng First People's Hospital

Language: English

Topic: Bone Metabolism and Diseases

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W3120831112

FWCI: 0.571

Cites: 24

Cited by: 10

Related to: 10