Osmotic regulation of aquaporin-8 expression in retinal pigment epithelial cells in vitro: Dependence on KATP channel activation.
Year: 2020
Type: article
Abstract: Purpose: gene expression and the localization of AQP8 in cultured human RPE cells. Methods: . Gene expression was determined with real-time RT-PCR analysis. AQP8 protein localization and secretion of VEGF were evaluated with immunocytochemistry, western blotting, and enzyme-linked immunosorbent assay (ELISA). Results: caused increases in hypoxic VEGF gene expression and secretion and decreased cell viability under control, hyperosmotic, and hypoxic conditions. Conclusions: channels. The data suggest that AQP8 activity decreases the hypoxic VEGF expression and improves the viability of RPE cells which may have impact for ischemic retinal diseases like diabetic retinopathy and age-related macular degeneration.
Source: PubMed
Authors: Benjamin Schnabel, Heidrun Kuhrt, Peter Wiedemann, Andreas Bringmann, Margrit Hollborn
Institutions: University Hospital Leipzig, Leipzig University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3122818365