Meiosis-specific ZFP541 repressor complex promotes meiotic prophase exit during spermatogenesis
Year: 2021
Type: preprint
Abstract: Summary During spermatogenesis, meiosis is accompanied by robust alteration in gene expression and chromatin status. However, it remained elusive how meiotic transcriptional program is established to ensure completion of meiotic prophase. Here, we identified a novel protein complex consisting of germ-cell-specific zinc-finger protein ZFP541 and its interactor KCTD19 as the key transcriptional regulator for meiotic prophase exit. Our genetic study showed that ZFP541 and KCTD19 are co-expressed from pachytene onward and play an essential role in the completion of meiotic prophase program in the testis. Furthermore, our ChIP-seq and transcriptome analyses revealed that ZFP541 binds to and suppresses a broad range of genes whose function is associated with biological processes of transcriptional regulation and covalent chromatin modification. The present study demonstrated that germ-cell specific ZFP541-KCTD19 containing complex promotes meiotic prophase exit in males, and triggers reconstruction of the transcription network and chromatin organization leading to post-meiotic development.
Authors: Yuki Horisawa-Takada, Chisato Kodera, Kazumasa Takemoto, Akihiko Sakashita, Kenichi Horisawa, Ryo Maeda, Shingo Usuki, Sayoko Fujimura, Naoki Tani, Kumi Matsuura, Ryuki Shimada, Tomohiko Akiyama, Atsushi Suzuki, Hitoshi Niwa, Makoto Tachibana, Takashi Ohba, Hidetaka Katabuchi, Satoshi H. Namekawa, Kimi Araki, Kei-ichiro Ishiguro
Language: English
Subfield: Reproductive Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funders: Naito Foundation, Sumitomo Foundation, Uehara Memorial Foundation, Ministry of Education, Culture, Sports, Science and Technology, University of Tokyo, Astellas Pharma, University of Tokushima, Daiichi Sankyo Foundation of Life Science, Astellas Foundation for Research on Metabolic Disorders, Japan Society for the Promotion of Science, Daiichi Sankyo Europe
Awards: G1850401843, Screening of germ cell related genes and generation of disease model animals, Study on the difference in chromatin domain between germ and somatic cells, G3111881881, G338994770, G345166697, Study on cell cycle regulation in meiosis I, Molecular mechanisms that make differences between mitosis and meiosis, G697504518, Mechanism of chromatin higher-order structural conversion during meiosis initiation in mammalian germ cells, Advanced Animal Model Support
OpenAlex ID: W3125814275