Work

Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue

Year: 2021

Type: article

Abstract: Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types. FACS has many challenges that limit comprehensive cell purification from the brain, leading to incomplete molecular characterization. Here, we present the intranuclear immunostaining-based FACS protocol with several modified steps, which allows optimized nuclei/cell sorting from mouse or human embryonic cortical tissue for distinct downstream molecular investigation of basal intermediate progenitors.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2021.100318

Authors: M. Sadman Sakib, Godwin Sokpor, Huu Phuc Nguyen, Andre Fischer, Tran Tuoc

Institutions: German Center for Neurodegenerative Diseases, Ruhr University Bochum, Multiscale Bioimaging

Language: English

Topic: Neurogenesis and neuroplasticity mechanisms

Subfield: Developmental Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,500

Funders: Department of Environment and Primary Industries, Deutsche Forschungsgemeinschaft, Deutsches Zentrum für Neurodegenerative Erkrankungen