Work

METTL3-dependent N 6 -methyladenosine RNA modification mediates the atherogenic inflammatory cascades in vascular endothelium

Year: 2021

Type: article

Abstract: Significance Using in vitro and in vivo approaches, this report demonstrated the increased METTL3 expression and m 6 A hypermethylation in response to disturbed flow and oscillatory shear stress. METTL3 hypermethylates m 6 A sites at various downstream targets, up-regulates NLRP1 and down-regulates KLF4, and elicits atherogenic responses. Collectively, METTL3 and m 6 A hypermethylation epigenetically regulates the atherogenic gene expression and phenotypes in the initiation of atherosclerosis. Our data may enlighten the field of RNA epigenetics in the pathogenic mechanisms of heart diseases.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.2025070118

Authors: Chian-Shiu Chien, Julie Yi-Shuan Li, Yueh Chien, Mong-Lien Wang, Aliaksandr A. Yarmishyn, Ping-Hsing Tsai, Chi-Chang Juan, Phu Nguyen, Hao-min Cheng, Teh-Ia Huo, Shih-Hwa Chiou, Shu Chien

Institutions: Taipei Veterans General Hospital, University of California San Diego, National Yang Ming University, Genomics Research Center, Academia Sinica, Academia Sinica

Language: English

Topic: RNA modifications and cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Ministry of Science and Technology, Taiwan, University of California, San Diego, National Heart, Lung, and Blood Institute

Awards: G1182363317, Nucleolin Regulation of miRome by Shear Stress, G1944143327, Systems Biology Analyses for Hemodynamic Regulation of Vascular Homeostasis, G4703009914, G6305034020, G8101276401, Integration of single-cell imaging and multi-omics sequencing to study EC mechano-pathophysiology

OpenAlex ID: W3127283963

FWCI: 7.152

Cites: 36

Cited by: 165

Related to: 10