Work

Generation of Sex-Reversed Female Clonal Mice via CRISPR-Cas9-Mediated Y Chromosome Deletion in Male Embryonic Stem Cells

Year: 2021

Type: article

Abstract: Mice derived entirely from embryonic stem (ES) cells can be generated through tetraploid complementation. Although XY male ES cell lines are commonly used in this system, occasionally, monosomic XO female mice are produced through spontaneous Y chromosome loss. Here, we describe an efficient method to obtain monosomic XO ES cells by CRISPR-Cas9-mediated deletion of the Y chromosome, allowing generation of female clonal mice by tetraploid complementation. The monosomic XO female mice are viable and able to produce normal male and female offspring. Direct generation of clonal mice in both sexes can significantly accelerate the production of complex genetically modified mouse models.

Source: The CRISPR Journal

DOI: 10.1089/crispr.2020.0074

Authors: Yiren Qin, Bokey Wong, Liangwen Zhong, Fuqiang Geng, Luis F. Parada, Duancheng Wen

Institutions: Weill Cornell Medicine, Memorial Sloan Kettering Cancer Center

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences

Awards: Direct generation of complex genetically-modified mouse models via embryonic stem cells, Isolation, characterization and translational development of glioma stem cells, H3.3-mediated epigenetic regulation of developmental bivalent genes for reprogramming and differentiation, Cancer Center Support Grant