Chinese medicine PaBing-II protects human iPSC-derived dopaminergic neurons from oxidative stress
Year: 2024
Type: article
Abstract: BackgroundPaBing-II Formula (PB-II) is a traditional Chinese medicine developed to treat Parkinson's disease (PD). However, due to the complexity of PB-II and the difficulty of culturing human dopaminergic neurons (DAn) in vitro, the mechanism of PB-II to treat PD remains unclear. MethodsWe established the human induced pluripotent stem cells (iPSCs) and derived DAn from hiPSCs to study the protective effects of PB-II on DAn after oxidative stress, which plays an important role in PD pathogenesis. ResultsWe found that serum derived from rats that had ingested PB-II significantly protect hiPSC-derived DAn from reactive oxygen species (ROS). In addition, PB-II dependent serum can activate nuclear erythroid-derived factor 2 (Nrf2) responses, which are required for the neutralization of ROS. In addition, PB-II can activate the Nrf2/ARE signal pathway of midbrain dopaminergic neurons of PD rats induced with 6-hydroxydopamine (6-OHDA) injury, rescue DAn cells, and improve the symptoms of PD rats. ConclusionsPB-II significantly protects the DA neurons from oxidative stress by activating the Nrf2 pathway.
Source: Frontiers in Immunology
Institutions: Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Guangzhou Women and Children Medical Center, Guangzhou Medical University, Fujian Agriculture and Forestry University, Second Affiliated Hospital of Zhejiang University, Zhejiang University
Language: English
Subfield: Clinical Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,295
OpenAlex ID: W4401277567