Proximity proteomics in a marine diatom reveals a putative cell surface-to-chloroplast iron trafficking pathway
Year: 2021
Type: article
Abstract: Iron is a biochemically critical metal cofactor in enzymes involved in photosynthesis, cellular respiration, nitrate assimilation, nitrogen fixation, and reactive oxygen species defense. Marine microeukaryotes have evolved a phytotransferrin-based iron uptake system to cope with iron scarcity, a major factor limiting primary productivity in the global ocean. Diatom phytotransferrin is endocytosed, however proteins downstream of this environmentally ubiquitous iron receptor are unknown. We applied engineered ascorbate peroxidase APEX2-based subcellular proteomics to catalog proximal proteins of phytotransferrin in the model marine diatom Phaeodactylum tricornutum . Proteins encoded by poorly characterized iron-sensitive genes were identified including three that are expressed from a chromosomal gene cluster. Two of them showed unambiguous colocalization with phytotransferrin adjacent to the chloroplast. Further phylogenetic, domain, and biochemical analyses suggest their involvement in intracellular iron processing. Proximity proteomics holds enormous potential to glean new insights into iron acquisition pathways and beyond in these evolutionarily, ecologically, and biotechnologically important microalgae.
Source: eLife
DOI: 10.7554/elife.52770
Institutions: Harvard University, Scripps Institution of Oceanography, University of California San Diego, Wyss Institute for Biologically Inspired Engineering, J. Craig Venter Institute, University of South Bohemia in České Budějovice, Institute of Parasitology, Czech Academy of Sciences, Biology Centre
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Life below water
Open Access status: gold
Estimated APC paid: $2,500
Funders: National Science Foundation, U.S. Department of Energy, Gordon and Betty Moore Foundation, Grantová Agentura České Republiky, National Institutes of Health, European Regional Development Fund, National Institute of General Medical Sciences, National Institute of Environmental Health Sciences, Biological and Environmental Research, European Social Fund
Awards: G12500569, Defining the Competitive Edge: Cellular Systems that Enable Nitrate Assimilation in Marine Diatoms, G176604203, Andrew Allen Investigator Award, G251319137, G291036901, G2934980467, G3490171961, Evolution and fate of endosymbiotic organelles in stramenopiles, G3979014076, G4131035492, G4192211598, Collaborative Research: Iron Bioavailability in High-CO2 Oceans: New Perspectives on Iron Acquisition Mechanisms in Diatoms, New Genetic Tools for Marine Diatoms, G5481527552, Cellular and transcriptional regulation of the seafood toxin domoic acid, G5916014575, G6404302932, New Genetic Tools for Marine Diatoms, G6963294263, G700989975, G7315588729, G7363506711, G7689605238, Photoparasitism in chromerid algae and dinoflagellates, DESIGN, SYNTHESIS, AND VALIDATION: GENOME SCALE OPTIMIZATION OF ENERGY FLUX THROUGH COMPARTMENTALIZED METABOLIC NETWORKS IN A MODEL PHOTOSYNTHETIC EUKARYOTIC MICROBE
OpenAlex ID: W3129792723