Year: 2021
Type: article
Abstract: Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and command-line tool for the design of PE experiments. PrimeDesign can be used for single and combination editing applications, as well as genome-wide and saturation mutagenesis screens. Using PrimeDesign, we construct PrimeVar, a comprehensive and searchable database that includes candidate prime editing guide RNA (pegRNA) and nicking sgRNA (ngRNA) combinations for installing or correcting >68,500 pathogenic human genetic variants from the ClinVar database. Finally, we use PrimeDesign to design pegRNAs/ngRNAs to install a variety of human pathogenic variants in human cells.
Source: Nature Communications
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,560
Funders: Howard Hughes Medical Institute, U.S. Department of Health and Human Services, Deutsche Forschungsgemeinschaft, National Institutes of Health, National Human Genome Research Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Biomedical Imaging and Bioengineering
Awards: Discovering disease-causal variants by linking genetic and epigenetic variation, Graduate Training in Computational and Systems Biology, Entwicklung hochspezifischer DNS Baseneditoren zur programmierbaren GenomEditierung, Expanding the Scope of Base Editing, G4606254678, Evolution, Optimization, and Application of Genome Editing Technologies, G6855349143, Multiscale exploration of the functional non-coding genome, Continuous Evolution of Proteins with Novel Therapeutic Potential, G7763705010, Integrating Chemistry and Evolution to Illuminate Biology and Enable Novel Therapeutics, Center for Genomic Editing and Recording: Development and Application of Next-Generation Genome and Epigenome Editing Methods to Advance the Study and Treatment of Human Disease, G995254689
OpenAlex ID: W3131093799