An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolution
Year: 2021
Type: article
Abstract: ABSTRACT Neural crest cells (NCCs) are vertebrate stem cells that give rise to various cell types throughout the developing body in early life. Here, we utilized single-cell transcriptomic analyses to delineate NCC-derivatives along the posterior developing vertebrate, zebrafish, during the late embryonic to early larval stage, a period when NCCs are actively differentiating into distinct cellular lineages. We identified several major NCC/NCC-derived cell-types including mesenchyme, neural crest, neural, neuronal, glial, and pigment, from which we resolved over three dozen cellular subtypes. We dissected gene expression signatures of pigment progenitors delineating into chromatophore lineages, mesenchyme subtypes, and enteric NCCs transforming into enteric neurons. Global analysis of NCC derivatives revealed they were demarcated by combinatorial hox gene codes, with distinct profiles within neuronal cells. From these analyses, we present a comprehensive cell-type atlas that can be utilized as a valuable resource for further mechanistic and evolutionary investigations of NCC differentiation.
Source: eLife
DOI: 10.7554/elife.60005
Institution: Rice University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,500
Awards: CAREER: Understanding spatiotemporal construction of vertebrate enteric nervous system, Defining the functional role of Bone Morphogenic Protein Signaling Pathway in the formation of the enteric nervous system, G863484009, Recruitment of First-Time, Tenure-Track Faculty Members, Cancer Center Support Grant, Baylor College of Medicine Cancer Center - Cancer Center Support Grant
OpenAlex ID: W3132264878