Work

Chemical modifications of proteins and their applications in metalloenzyme studies

Year: 2021

Type: review

Abstract: Protein chemical modifications are important tools for elucidating chemical and biological functions of proteins. Several strategies have been developed to implement these modifications, including enzymatic tailoring reactions, unnatural amino acid incorporation using the expanded genetic codes, and recognition-driven transformations. These technologies have been applied in metalloenzyme studies, specifically in dissecting their mechanisms, improving their enzymatic activities, and creating artificial enzymes with non-natural activities. Herein, we summarize some of the recent efforts in these areas with an emphasis on a few metalloenzyme case studies.

Source: Synthetic and Systems Biotechnology

DOI: 10.1016/j.synbio.2021.01.001

Authors: Nathchar Naowarojna, Ronghai Cheng, Juan Lopez, Christina Wong, Lu Qiao, Pinghua Liu

Institution: Boston University

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,500

Funder: National Science Foundation