Mechanism of Near-Infrared Fluorescent Signal Generation in Proteolysis-Sensitive Macromolecular Imaging Probes
Year: 2013
Type: article
Abstract: In vivo optical imaging of proteolysis in inflammatory and tumor microenvironments can be monitored through excitable fluorophore-labeled probes using a graft copolymer of poly(ethylene glycol) and poly-L-lysine as a carrier molecule. In this project, both in vitro and cell culture experiments were performed to investigate the major mechanisms of PGC-fluorophore probe cleavage via stromal cell proteases. It was observed that trypsin-mediated cleavage of PGC-fluorophore probes results in distinct fragment sizes, a 2-fold increase in fluorescence intensity, and a rapid 1.5-fold increase in fluorescence lifetime. Finally, it was observed that human squamous carcinoma cells uptake probes faster than normal human dermal fibroblasts with a greater fluorescence intensity over time.
Source: Digital WPI
Author: Jessica Clotilde Lopez
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W3133641843