Work

Glycoengineering Chinese hamster ovary cells: a short history

Year: 2021

Type: review

Abstract: Biotherapeutic glycoproteins have revolutionised the field of pharmaceuticals, with new discoveries and continuous improvements underpinning the rapid growth of this industry. N-glycosylation is a critical quality attribute of biotherapeutic glycoproteins that influences the efficacy, half-life and immunogenicity of these drugs. This review will focus on the advances and future directions of remodelling N-glycosylation in Chinese hamster ovary (CHO) cells, which are the workhorse of recombinant biotherapeutic production, with particular emphasis on antibody products, using strategies such as cell line and protein backbone engineering.

Source: Biochemical Society Transactions

DOI: 10.1042/bst20200840

Authors: Roberto Donini, Stuart M. Haslam, Cleo Kontoravdi

Institution: Imperial College London

Language: English

Topic: Viral Infectious Diseases and Gene Expression in Insects

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funders: Directorate for Biological Sciences, Biotechnology and Biological Sciences Research Council