Year: 2019
Type: preprint
Abstract: Summary: This protocol describes a method to mount live 8 day-old zebrafish larvae in agarose for capturing fluorescent transgene-expressing peripheral somatosensory axons with confocal imaging. We have previously described a similar method in the Journal of Visualized Experiments (1). Diabetic Complication: References: 1. G. S. O'Brien, S. Rieger, S. M. Martin, A. M. Cavanaugh, C. Portera-Cailliau, A. Sagasti, Twophoton axotomy and time-lapse confocal imaging in live zebrafish embryos. J Vis Exp, (2009). 2. S. Rieger, A. Sagasti, Hydrogen peroxide promotes injury-induced peripheral sensory axon regeneration in the zebrafish skin. PLoS Biol 9, e1000621 (2011); published online EpubMay (10- PLBI-RA-9260R3 [pii] 10.1371/journal.pbio.1000621). 3. A. J. Pittman, M. Y. Law, C. B. Chien, Pathfinding in a large vertebrate axon tract: isotypic interactions guide retinotectal axons at multiple choice points. Development 135, 2865-2871 (2008); published online EpubSep
Author: Sandra Rieger
Institution: University of Miami
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3133795575