Work

Zona pellucida removal using Acid Tyrode solution and snap freezing v1

Year: 2019

Type: preprint

Abstract: A simple protocol to remove the zona pellucida from bovine embryos. This is a necessary step before freezing embryos for analysis of RNA or DNA because of contaminating cumulus and sperm cells associaed with the zona pellucida.

DOI: 10.17504/protocols.io.6kwhcxe

Authors: Elizabeth Jannaman, Peter J Hansen

Institution: University of Florida

Language: English

Topic: Reproductive Biology and Fertility

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

Open Access status: green

OpenAlex ID: W3133961142

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