Year: 2019
Type: preprint
Abstract: Summary: To induce hyperglycemia in larval zebrafish, utilize a genetic β-cell ablation system involving the Tg(ins:NTR-mCherry) transgenic zebrafish strain (courtesy of Dr. Michael Parsons, Johns Hopkins University). This strain expresses the bacterial enzyme Nitroreductase (NTR), which is encoded by the nfsB gene, in β-cells of the pancreas starting around 24 hours post fertilization (hpf). Because NTR is genetically fused to the red fluorescent protein mCherry, β-cells expressing NTR can be visualized with fluorescence microscopy. Upon addition of the prodrug metronidazole (MTZ) to the embryo medium, NTR converts MTZ into a toxin, which selectively ablates NTR-expressing β-cells (Curado et al., Nature Protocols 3, 948-954 (2008). This can be monitored by the disappearance of the fluorescence under a confocal microscope. Diabetic Complication:
Author: Sandra Rieger
Institution: University of Miami
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W3134853294