Work

Quantifying Biogenic Silica (bSi) Deposition Rates Adapted Method & Fluorescence Reading (PDMPO) via Fluorometer v1

Year: 2019

Type: preprint

Abstract: This method can be used to assess and quantify the rate of silica deposition (bSi) over time in diatoms to determine their rate of frustule synthesis. This protocol has been adapted for the processing of both cultures as well as environmental samples when inoculated with PDMPO [2‐(4‐pyridyl)‐5‐((4‐(2dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole], which is a fluorescent dye that is co-deposited with silica during frustule biosynthesis in a 3230:1 Si:PDMPO (mol:mol) ratio.

DOI: 10.17504/protocols.io.735hqq6

Authors: Brittany N Zepernick, Matthew Saxton, Steven W Wilhelm

Institution: University of Tennessee at Knoxville

Language: English

Topic: Diatoms and Algae Research

Subfield: Ecology, Evolution, Behavior and Systematics

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W3135393957

Cites:

Cited by:

Related to: 10