Global Proteomics and Phosphoproteomics of Heart Failure Patients Elucidate Etiology-specific Signaling
Year: 2019
Type: dissertation
Study design: Observational Study
Abstract: The prognosis and treatment outcomes of heart failure patients rely on patient etiology yet the underlying signaling mechanisms are complex and not fully elucidated. Phosphorylation is a major form of protein regulation, profoundly altering signaling networks. Here, we identified disease-specific regulation of signaling pathways between dilated (DCM) and ischemic (ICM) cardiomyopathies as well as regional differences within infarcted hearts. Myocardial tissues were explanted from DCM patients, infarct, peri-, and non-infarct regions of ICM patients, and non-failing controls (NFC). Following phosphorylated peptide enrichment, peptide fractions were subjected to liquid chromatography-tandem mass spectrometry for global proteomic and phosphoproteomics profiling. Differential protein and pathway analyses were performed to determine protein expression, phosphorylation, and pathways that were significantly altered in pair-wise comparisons (DCM vs. NFC, infarct vs. non-infarct, and peri- vs. non-infarct). This comprehensive study presents novel findings on disease-specific regulation of molecular pathways in heart failure to reveal suitable targets for therapeutic interventions.
Source: TSpace (University of Toronto)
Author: Da Hye Kim
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W3136759857